rC5a (Human) Polyclonal Rabbit Antiserum

Whole polyclonal antiserum from rabbits immunized with highly purified human complement protein.

Brand

SKU: A221C Category:

Description

General Description
Product is whole polyclonal antiserum from rabbits immunized with highly purified human complement protein. Product is not a purified IgG fraction. Rabbits are maintained in FDA certified facilities.

Physical Characteristics & Structure
Antibodies present in the antisera are primarily IgG.

Applications
Recognizes rC5a, human C5a and human C5adesArg.
Western Blots: Effective at dilutions 1/500 to 1/1000 depending on conditions.
Most effective against non-reduced antigen.
ELISA: Effective at dilutions 1/500 to 1/2000 depending on conditions.
Immunodiffusion: Effective against 50 µg/ml C5a at 1/4 dilution.

Specifications

Sizes Available:           1.0 mL
Concentration:             >40 mg/mL total protein concentration.
Extinction Coeff.:        A280 nm = 1.0 at 1.0 mg/mL
Form:                            Frozen liquid, clear, yellow/tan colored.
Activity:                        1/4 dilution versus activated NHS assayed by immunodiffusion1/500 to 1/1000 effective concentrations Western Blots 1/500 to/2000 effective concentrations in ELISA.
Preservative:                0.09 % sodium azide.
Storage:                        -20o C or below (long term), +4o C (short term, <30 days)
Source:                          Rabbits (Oryctolagus cuniculus) immunized with highly purified human complement protein.
Specificity:                    Monospecific for C5a and C5adesArg in human plasma
Origin:                            Manufactured in the USA.

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