α-GST, Human Urine, Serum, Plasma

$379.00

Kidney Injury Biomarker for proximal tubular damage

Brand

SKU: TE1056 Category:

Description

Intended use: Alpha Glutathione S-Transferase (α-GST) is a member of the GST superfamily of small cytosolic proteins primarily involved in cellular detoxification reactions. α-GST is approximately 51kDa and can comprise up to 2 % of the cytosolic soluble protein content. As a result of its constitutive nature, α-GST is immediately released into the urine upon lysis of epithelial cells of the proximal tubule. The α-GST EIA provides a method for the quantitative determination of alpha glutathione S-transferase (α-GST) in human urine. The α-GST EIA can also be used for the determination of α-GST in human, serum and plasma, to assess liver injury. Elevated urinary α-GST levels are an early indicator of acute kidney injury (AKI) onset in cases of nephrotoxicity, environmental toxicity, cardiothoracic surgery and in transplantation rejection. The release of π-GST has been shown to be associated with distal tubular damage, thus simultaneous measurement of both α-GST and π-GST allows discrimination between proximal and distal tubular damage. Low basal levels of α-GST are released in normal healthy individuals.

Specifications

Range: 2.5 - 80 μg/l
Sensitivity: 1.9 μg/l
Incubation time: 2 hours
Sample volume: 100 μl
Sample type:

Urine (Suggested initial dilution 1:2)

 

Sample preparation:

Urine: (Suggested initial dilution 1:2)

Overnight or 24 h urine is recommended.
As soon as possible after collection sample should be diluted with urine stabilizing buffer (USB). Sample:USB 4:1. After the addition of USB, samples can be stored at 20-25 °C for up to 48 hours, at 2-8 °C for up to one week or at - 20 °C for >1 year.
Repeated freeze thawing of samples should be avoided.

Serum, Plasma: (Suggested initial dilution 1:5)

Centrifuge within 3 hours from time of collection and transfer the sample from the original tube for storage. Samples can be stored at 20 – 25 °C for up to 48 hours, at 2 – 8 °C for up to one week or at -20 °C for >1 year. Repeated freeze thawing of samples should be avoided.
Repeated freeze thawing of samples should be avoided.

Reference values:

Urine: 0 - 29.0 μg/l
Serum, Plasma: 0-12 μg/l

Species:

Human

Tests: 96
Method: ELISA
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