Description
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The assay set-up is performed in the same manner as the standard 3H-thymidine incorporation method. Instead of pulsing with tritiated nucleotide, 20 μl of dye solution is added to 200 μl sample. The incubation time is dependent on the metabolic capacity of the cells. Usually two to five hours of incubation at 37°C are sufficient to yield a significant increase in color intensity. As different cells vary in their ability to convert the yellow colored tetrazolium compound to its red formazan derivative, we recommend testing every new cell-line’s metabolic capacity as described in the figure below. Intended use: The EZ4U kit is a two to five-hour test for the measurement of cell proliferation and cell toxicity. The EZ4U test employs non-toxic tetrazolium salts, which are reduced to colored formazan. As this reduction process requires functional mitochondria, which are inactivated within a few minutes after cell death, this method provides an excellent tool for the discrimination between living and dead cells. Intended applications:
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