$100.00
CVF-NHS is generated by activating the complement system in normal human serum with cobra venom factor (CVF) (CompTech #A150) purified from the venom of Naja naja kaouthia.
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General Description
CVF-NHS is generated by activating the complement system in normal human serum with cobra venom factor (CVF) (CompTech #A150) purified from the venom of Naja naja kaouthia. CVF from Naja naja kaouthia produces an enzyme that cleaves both human C3 and C5. The affinity of the Naja naja kaouthia CVF for C5 is so high that it primarily consumes C5 first, then C3 ((Rawal, N. and Pangburn, M.K. (2000)).
Sizes Available: 1.0 ml/vial
Concentration: >50 mg/ml (see Certificate of Analysis for exact conc.)
Form: Frozen liquid
Activity: <10% C3 and C5 activity versus normal human serum standard
Buffer: None
Preservative: None, 0.22 µm filtered
Storage: -70o C or below. Minimize freeze/thaw cycles.
Source: Normal human serum (shown by certified tests to be negative for HBsAg and for antibodies to HCV, HIV-1 and HIV-II).
Precautions: Use normal precautions for handling human blood products.
Restrictions: Not available for sale outside the USA due to international endangered species laws.
Origin: Manufactured in the USA.
Precautions/Toxicity/Hazards
The source is human serum, therefore precautions appropriate for handling any blood-derived product must be used even though the source was shown by certified tests to be negative for HBsAg and for antibodies to HCV, HIV-1 and HIV-II.
Hazard Code: B WGK Germany 3
Physical Characteristics & Structure
CVF-NHS is serum supplied as a clear, straw-colored liquid containing an activated complement system and hence no complement activity. CVF-NHS also contains CVF used for activating the complement system.
Function
CVF-NHS is tested for C3 and C5 activity by hemolytic assays using antibody sensitized sheep erythrocytes (CompTech #B200) and C3-depleted human serum (Cat #A314) or C5-depleted human serum (Cat #A320). The assays verify that CVF-NHS has < 10% of C3 and C5 activity or >90% consumption of C3 and C5 activity in CVF-NHS when compared to normal human serum standard.
Assays
See Function
Applications
CVF-NHS is a serum source which lacks complement activity due to its consumption upon activation with CVF.