C5a/C5a des-arg (neo-epitope), Human, clone 2952

The monoclonal antibody 2952 recognizes a neo-epitope on human complement protein C5a/C5a des-Arg.

Brand

SKU: TE2079-100UG Category:

Description

The monoclonal antibody 2952 recognizes a neo-epitope on human complement protein C5a/C5a des-Arg. C5 is involved in the activation of the lytic pathway within the complement system which is an important factor in innate immunity. The activation pathways lead via C3 to the cleavage of the fifth complement component C5. During complement activation, C5 is proteolytically cleaved and the anaphylatoxic peptide C5a is generated. C5a is a small polypeptide consisting of 74 amino acids (~11 kDa) and is derived from the N-terminus of the αchain. C5a itself is very short-lived and in serum is cleaved rapidly into the more stable, though biologically still active C5a-desArg (also called acylation stimulating protein, ASP). C5a acts as a potent anaphylatoxin causing smooth muscle contraction, vasodilatation, increased vascular permeability, basophil and mast cell degranulation and lysosomal enzyme release. In addition, C5a is a potent chemotactic factor for neutrophils, eosinophils, basophils and monocytes. C5a is involved in inflammatory reactions seen in gram-negative bacterial sepsis, trauma, ischemic heart disease, post-dialysis syndrome and a variety of autoimmune diseases. Elevation of C5a is associated with increased cardiovascular risk in patients with advanced atherosclerosis. Also, C5a is closely associated with the capillary leak syndrome in leukemic children after bone marrow transplantation. C5a is also a marker in urine for predicting the onset of acute graft rejection after kidney transplantation. Monoclonal antibody 2952 is a subclone of clone 2925. Please be aware that under given conditions it is known that C5 can expose epitopes normally only found in the cleaved activation products (ref.5).

Specifications

Volume:                                      1 ml

Amount:                                     100 µg

Formulation:                              0.2 µm filtered in PBS+0.1%BSA

Concentration:                          100 µg/ml

Host Species:                            Mouse IgG1

Conjugate:                                 None

Endotoxin:                                 <24 EU/mg

Purification:                               Protein G

Storage:                                      4°C

Safety and Handling

Storage & stability

Product should be stored at 4°C. Under recommended storage conditions, product is stable for at least one year.

Precautions

For research use only. Not for use in or on humans or animals or for diagnostics. It is the responsibility of the user to comply with all local/state and federal rules in the use of this product. Hycult Biotech is not responsible for any patent infringements that might result from the use or derivation of this product.

Additional Information

Immunogen

ISHKDMQLG (C5a amino acid 65-73)

Cross reactivity

Human C5: No

What Makes Us Unique?

GSA MEDICAL portfolio offers a comprehensive workflow for your lab needs with wide range of tools.

As of 2020, GSA MEDICAL INC. started providing premier Manufacturing services to the diagnostic industry. GSA MEDICAL can effectively analyze the feasibility of reagents for use in rapid immunoassays. This process offers a quick glance as to the potential a reagent or concept may have and can result in saving considerable time and money for GSAMEDICAL’s clients.

We provide strong personal support, technical competence, and experience to ensure customer expectations will be met or exceeded.

Shop Products