Description
General Description
Product is whole polyclonal antiserum from goats immunized with highly purified human complement protein. Product is not a purified IgG fraction. Goats are maintained in FDA certified facilities.
Physical Characteristics & Structure
Antibodies present in the antisera are primarily IgG.
Applications
Western Blots: Effective at dilutions 1/4,000 to 1/8,000 depending on conditions.
Note: Most effective against non-reduced antigen.
ELISA: Effective at dilutions 1/8,000 to 1/16,000 depending on conditions.
Immunodiffusion: Effective against NHS and plasma at 1/8 dilution
Specifications
Sizes Available: 1.0 mL
Concentration: >40 mg/mL total protein concentration.
Extinction Coeff. A280 nm = 1.0 at 1.0 mg/mL
Form: Frozen liquid, clear, yellow/tan colored.
Activity: 1/8 dilution versus NHS in immunodiffusion 1/8,000 effective concentration in Western Blots 1/16,000 effective concentration in ELISA.
Preservative: 0.09 % sodium azide.
Storage: -20o C or below (long term), +4o C (short term, <30 days)
Source: Goats (Capra aegagrus hircus) immunized with highly purified human complement protein.
Specificity: Monospecific for C5 in human plasma and serum.
Origin: Manufactured in the USA.
