Description
General Description
Product is whole polyclonal antiserum from rabbits immunized with highly purified human complement protein. Product is not a purified IgG fraction. Rabbits are maintained in FDA certified facilities.
Physical Characteristics & Structure
Antibodies present in the antisera are primarily IgG.
Applications
Western Blots: Effective at dilutions 1/500 to 1/1000 depending on conditions.
Most effective against non-reduced antigen.
ELISA: Effective at dilutions 1/500 to 1/2000 depending on conditions.
Immunodiffusion: Effective against NHS (20 µg/ml C4a) at 1/4 dilution.
Specifications
Sizes Available: 1.0 mL
Concentration: >40 mg/mL total protein concentration.
Extinction Coeff. A280 nm = 1.0 at 1.0 mg/mL
Form: Frozen liquid, clear, yellow/tan colored.
Activity: 1/4 dilution versus activated NHS assayed by immunodiffusion 1/500 to 1/1000 effective concentrations in Western Blots 1/500 to 1/2000 effective concentrations in ELISA.
Preservative: 0.09 % sodium azide.
Storage: -20o C or below (long term), +4o C (short term, <30 days)
Source: Rabbits (Oryctolagus cuniculus) immunized with highly purified human complement protein.
Specificity: Monospecific for C4a in human plasma
Origin: Manufactured in the USA.
