Description
General Description
Product is made from polyclonal antiserum from goats immunized with highly
purified human complement protein. Product is the purified IgG fraction. Goats are
maintained in FDA certified facilities.
Physical Characteristics & Structure
Antibodies present are primarily IgG.
Applications
Western Blots: Effective at dilutions 1/4,000 to 1/8,000 depending on conditions.
Note: Most effective against non-reduced antigen.
ELISA: Effective at dilutions 1/8,000 to 1/16,000 depending on conditions.
Radial immunodiffusion: 1/44 dilution (Vol/Vol) of anti-C2 IgG fraction in agarose gel results, after staining, in clear precipitin rings with
neat, 1:2 and 1:4 dilutions of NHS (3 mm wells, 20 µl sample). versus activated ½ diluted NHS assayed by immunodiffusion and staining of the precipitin band.
Immunodiffusion: Effective at 1/8 dilution against NHS or plasma.